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Báo cáo khoa học: Hydrogen peroxide efflux from muscle mitochondria underestimates matrix superoxide production – a correction using glutathione depletion

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:13

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The production of H2O2 by isolated mitochondria is frequently used as a measure of mitochondrial superoxide formation. Matrix superoxide dismu-tase quantitatively converts matrix superoxide to H2O2. However, matrix enzymes such as the glutathione peroxidases can consume H2O2 and com-pete with efflux of H2O2 , causing an underestimation of superoxide produc-tion.

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Nội dung Text: Báo cáo khoa học: Hydrogen peroxide efflux from muscle mitochondria underestimates matrix superoxide production – a correction using glutathione depletion

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