
Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii
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Transcriptional activator VnfA is required for the expression of a second nitrogenase system encoded in thevnfHandvnfDGKoperons inAzotobac-ter vinelandii. In the present study, we have purified full-length VnfA pro-duced inE. colias recombinant proteins (Strep-tag attached and tag-less proteins), enabling detailed characterization of VnfA for the first time.
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