Capillary Electrophoresis
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LIF (laser-induced fluorescence) is a very popular CE detector – These have ~0.01 attomole sensitivity for fluorescent molecules (e.g. derivatized proteins). Direct absorbance (UV-Vis) can be used for organics. For inorganics, indirect absorbance methods are used instead, where a absorptive buffer (e.g. chromate) is displaced by analyte ions – Detection limits are in the 50-500 ppb range. Alternative methods involving potentiometric and conductometric detection are also used – Potentiometric detection: a broad-spectrum ISE – Conductometric detection: like IC...
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