Further Study on Chemical Constituents of Croton tonkinensis Gagnep., Euphorbiaceae

Journal of Chemistry, Vol. 41, No. 1, P. 1, 2003<br /> <br /> <br /> short communication:<br /> <br /> Further Study on Chemical Constituents of<br /> Croton tonkinensis Gagnep., Euphorbiaceae<br /> Received 15-2-2003<br /> Phan Minh Giang , Jung Joon Lee2, Phan Tong Son1<br /> 1<br /> <br /> 1<br /> Faculty of Chemistry, Vietnam National University, Hanoi<br /> 2<br /> Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology<br /> <br /> Croton tonkinensis Gagnep., commonly and ent-7 -hydroxy-15-oxokaur-16-en-18-yl<br /> named in Vietnamese as "Kho sam Bac Bo", is a<br /> acetate (5.6%). The phytosterols -sitosterol,<br /> tropical shrub native to the Northern Vietnam. Its<br /> stigmasterol and two long chain alkyl alcohols<br /> dried leaves (Folium Tonkinensis) have been used<br /> were also isolated from the non-polar parts of the<br /> in Vietnamese traditional medicine to treat boils,<br /> methanol extract.<br /> abscesses, impetigo, abdominal pain, dyspepsia,<br /> dysentery, gastric and duodenal ulcers [1, 2]. The<br /> Acknowledgements: The authors are grateful to<br /> plant is of our interest since its varied biological<br /> activities demonstrated in our various in vitro International Foundation for Science (IFS,<br /> bioassays including anti-inflammatory, anti- Stockholm, Sweden) and the National Basic<br /> cancer, antiplasmodial, antibacterial and Research Program in Natural Sciences for the<br /> antifungal. financial support for this work.<br /> Previously we reported on the isolation and References<br /> structural determination of a new diterpenoid ent-<br /> 7 -hydroxy-15-oxokaur-16-en-18-yl acetate [3,4] 1. Vo Van Chi. Dictionary of Vietnamese<br /> from the ethanol extract of the leaves of C. Medicinal Plants, Medicine Publishing<br /> tonkinensis. Investigation of the methanol extract House, HCM City, P. 622 - 623 (1997).<br /> of the leaves of C. tonkinensis by HPLC coupled 2. Selected Medicinal Plants in Vietnam.<br /> with PDA detector revealed the presence of 3 Science and Techniques Publishing House,<br /> main flavonoids (in total 60.43% of the extract) Hanoi, Vol. 1, P. 260 - 262 (1999).<br /> one of which was isolated and structurally 3. Phan Tong Son, Van Ngoc Huong, Phan Minh<br /> identified as tiliroside on the basis of ESIMS, 1H Giang, Walter C. Taylor. Vietnam J. Chem.,<br /> NMR, 13C NMR spectra [5]. The other Vol. 37, No. 4, P. 1 - 2 (1999).<br /> constituents of the extract are diterpenoids<br /> 4. Phan Tong Son, Phan Minh Giang, Walter C.<br /> (12.5%) among which four ent-kaurane-type<br /> Taylor. Aust. J. Chem., Vol. 53, P. 1003 -<br /> diterpenoids were isolated and structurally<br /> 1005 (2000).<br /> characterized on the basis of ESIMS, FABMS, 1D<br /> 5. K. Y. Jung, S. R. Oh, S. H. Park, I. S. Lee, K.<br /> and 2D NMR including two new ent-1 -acetoxy-<br /> S. Ahn, J. J. Lee, and H. K. Lee. Biol. Pharm.<br /> 7 ,14 -dihydroxykaur-16-en-15-one (1.26%) Bull., Vol. 21, P. 1077 - 1078 (1998).<br /> and ent-18-acetoxy-7 ,14 -dihydroxykaur-16- 6. N. B. Perry, E. J. Burgess, S. H. Baek, R. T.<br /> en-15-one (1.22%) together with two known ent-7 Weavers, W. Geis, A. B. Mauger.<br /> ,14 -dihydroxykaur-16-en-15-one (0.57%) [6] Phytochem., Vol. 50, P. 423 - 433 (1999).<br /> <br /> <br /> 1<br /> 2<br />