A virus generation

Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to a identify, manipulate DNA, detect infectious organisms, including the viruses that cause AIDS, hepatitis, tuberculosis, detect genetic variations, including mutations, in human genes and numerous other tasks.

62p zingzing09 24-04-2013 59 7   Download

 An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation established an alternative reverse genetics system for influenza virus generation by using an adenovirus vector (AdV)  which achieves highly efficient gene transfer independent of cell transfection efficiency.

4p thuyancn 01-06-2015 33 4   Download

An improved reverse genetics system for influenza A virus generation and its implications for vaccine production present an improved system for the generation of influenza viruses that allows the easy and repro ducible production of vaccine viruses in vero cells. Application of this system may be especially advantageous in situations of outbreaks of highly pathogenic avian influenza viruses.

5p thuyancn 01-06-2015 39 4   Download

Type I interferons (IFN) are important components of the innate antiviral response. A key signalling pathway activated by IFNais the Janus kinase⁄ signal transducer and activator of transcription (JAK⁄STAT) pathway. Major components of the pathway have been identified. However, critical kinetic properties that facilitate accelerated initiation of intracellular antiviral signalling and thereby promote virus elimination remain to be determined.

14p viettel02 19-02-2013 40 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Polymerase activity of hybrid ribonucleoprotein complexes generated from reassortment between 2009 pandemic H1N1 and seasonal H3N2 influenza A viruses

15p dauphong1 21-12-2011 40 4   Download

RNA interference (RNAi) is a powerful tool to silence gene expression post-transcriptionally. Delivering sequences of RNAi in vivo remains a problem. The aim of this study was to use JC virus (JCV) virus-like particles (VLPs) as a vector for delivering RNAi in silencing the cytokine gene of IL-10. Methods: JCV VLPs were generated by recombinant JCV VP1 protein in yeast expression system. DNA fragment containing IL-10 shRNA was packaged into VLPs by osmotic shock. Results: In RAW 264.7 cells, IL-10 shRNA was found to reduce IL-10 expression by 85 to 89%, as compared with VLPs alone.

9p toshiba23 18-11-2011 61 3   Download