Alternative transcripts
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Transcripts with alternative 5′-untranslated regions (UTRs) result from the activity of alternative promoters and they can determine gene expression by influencing its stability and translational efficiency, thus executing complex regulation of developmental, physiological and pathological processes.
9p vielonmusk 21-01-2022 10 0 Download
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Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains.
10p thiencuuchu 27-11-2021 9 1 Download
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Hepatopoietin (HPO) is a novel human hepatotrophic growth factor. Recently, we demonstrated that the extra-cellular ligand form of HPO can stimulate proliferation of hepatic cells via its specific receptor, which is on the surface of these cells.Also, the intracellular formofHPOpotentiates the transcriptional factor AP-1. Intriguingly, a variety of HPO complexes with different molecular masses were detected in hepatocytes.
6p research12 23-04-2013 24 4 Download
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During starvation induced encystment, cells of the myxo-myceteDidymiumiridisaccumulate a7.5-kbRNAthat is the result of alternative processing of pre-rRNA. The 5¢ end corresponds to an internal processing site cleaved by the group I-like ribozyme DiGIR1, located within the twin-ribozyme intron Dir.S956-1. The RNAretains the majority ofDir.S956-1 including thehoming endonuclease geneanda small spliceosomal intron, the internal transcribed spacers ITS1 and ITS2, and the large subunit rRNAlacking its two group I introns. ...
9p tumor12 22-04-2013 28 1 Download
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E2F transcription factors play an important role in the regulation of cell cycle progression. E2F6, the most recently identified member of the E2F family, is a retinoblastoma-protein-independent transcriptional repressor that is required for developmental patterning of the axial skeleton. It has recently been shown that theE2f6locus produces two different mRNAs, E2F6 and E2F6b. The E2F6b mRNA contains an additional exon that is inserted by alternative splicing.
7p tumor12 22-04-2013 31 1 Download
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We have determined the cDNA nucleotide sequence, deduced the amino acid sequence and defined the gene structure for the cellular heart-type (H-FABP) or fatty acid-binding protein 3 (FABP3) from zebrafish. The zebrafish FABP3 exhibited the greatest amino acid sequence identity to fish andmammalian heart-type FABPs. 3¢RACE and 5¢ RLM-RACE mapped two alternative polyadenylation sites and three transcription start sites, respectively.
12p tumor12 20-04-2013 34 2 Download
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PSA (prostate-specific antigen), the most useful serum marker for prostate cancer, is encoded by thehKLK3gene and is present in the serumas amixture of several molecular species. This work was performed to identify the hKLK3 transcripts in order to determine how many proteins resembling PSA are synthesized from thehKLK3gene and secreted in blood. Combined Northern blotting, molecular cloninganddatabase searching showed that thehKLK3gene produces at least 15 transcripts ranging in size from 0.7 to 6.1 kb. Polysomal distribution analysis revealed that the transcripts shorter than 3.
9p tumor12 20-04-2013 50 1 Download
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TheRel/NF-jBfamilyof transcription factorsplaykey roles in morphogenesis and immune responses. We reported previously that As-rel1 and As-rel2 of the ascidianHalo-cynthia roretziare involved in notochord formation. The As-rel1 protein is a typical Rel/NF-jB family member, whereas the As-rel2 protein is a novel truncated product of As-rel1 that lacks a nuclear localization signal and the unique C-terminal region. Here, we present conclusive evi-dence that As-rel1 and As-rel2 are generated from a single gene by alternative splicing....
10p fptmusic 12-04-2013 38 1 Download
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We have identifiedDNA sequences required for the expres-sion of thepresenilin 1(PS1) gene. A promoter region has been mapped in SK-N-SH cells and includes sequences be-tween)118and+178flankingthemajor initiationsite (+1). ThePS1gene is also efficiently transcribed in the SH-SY5Y subclone of SK-N-SH cells. However the promoter appears tobeutilized inalternativeways inbothcell types. Sequences both upstream as well as downstream from the initiation site mapped in SK-N-SH cells were shown by 5¢-and3¢-deletion analysis to play a crucial role in both cell lines....
10p awards 05-04-2013 24 3 Download
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Báo cáo khoa học: Regulation of STAT signalling by proteolytic processing Lisa Hendry and Susan John
Interaction of cytokines with their cognate receptors leads to the activation of latent transcription factors, the signal transducer and activator of transcription (STAT) proteins. Numerous studies have identified the critical roles played by STAT proteins in regulating cell proliferation, differ-entiation and survival. Consequently, the activity of STAT proteins is negatively regulated by a variety of different mechanisms, which include alternative splicing, covalent modifications, protein–protein interactions with negative regulatory proteins and proteolytic processing by pro-teases....
8p awards 05-04-2013 37 3 Download
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We recently cloned a novel osmotic stress transcription factor 1 (OSTF1) from gills of euryhaline tilapia (Oreochromis mossambicus) and demonstra-ted that acute hyperosmotic stress transiently increases OSTF1 mRNA and protein abundance [Fiol DF, Ku¨ltz D (2005) Proc Natl Acad Sci USA 102, 927–932].
16p inspiron33 23-03-2013 33 2 Download
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Eph receptor tyrosine kinases regulate many important biological pro-cesses. In the present study, we explored the substrate specificity of the EphA4 receptor tyrosine kinase using peptide arrays. We define a consen-sus substrate motif for EphA4 and go on to identify and test a number of potential EphA4 substrates and map their putative site(s) of phosphoryla-tion.
11p media19 06-03-2013 45 3 Download
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Alternative splicing of the locus AbH-J-J generates functionally distinct proteins: the enzyme aspartyl (asparaginyl) b-hydroxylase, humbug and junctate (truncated homologs of aspartyl (asparaginyl) b-hydroxylase with a role in calcium regulation), and junctin (a structural protein of the sarco-plasmic reticulum membrane).
15p media19 04-03-2013 21 2 Download
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Several thousand plant genes are known to produce multiple transcripts, but the precise function of most of the alternatively encoded proteins is not known. Alternative splicing has been reported for the H-protein subunit of glycine decarboxylase in the genus Flaveria. H-protein has no catalytic activity itself but is a substrate of the three enzymatically active subunits, P-, T- and L-protein.
7p viettel02 20-02-2013 33 2 Download
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Thec-myc promoter-binding protein-1 (MBP-1) is a transcriptional sup-pressor of tumorigenesis and thought to be the product of alternative translation initiation of the a-enolase (ENO1) transcript. In the present study, we cloned a 2552-bp novel cDNA with a putative coding sequence ofMBP-1and functionally examined its ability to encode the MBP-1 pro-tein. Similarly to ENO1, the obtained MBP-1was widely and differentially expressed in a variety of normal tissues and cancer cells.
14p viettel02 19-02-2013 36 2 Download
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Alternate DNA structures that deviate from B-form double-stranded DNA such as G-quadruplex (G4) DNA can be formed by sequences that are widely distributed throughout the human genome. G-quadruplex secondary structures, formed by the stacking of planar quartets composed of four guanines that interact by Hoogsteen hydrogen bonding, can affect cellular DNA replication and transcription, and influence genomic stability.
19p viettel02 19-02-2013 29 3 Download
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TIA-1-related (TIAR) protein is a shuttling RNA-binding protein impli-cated in several steps of RNA metabolism. In the nucleus, TIAR contrib-utes to alternative splicing events, whereas, in the cytoplasm, it acts as a translational repressor on specific transcripts such as adenine and uridine-rich element-containing mRNAs.
19p mobifone23 21-01-2013 55 5 Download
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Hepatitis B virus (HBV) transcripts are subjected to multiple splicing deci-sions, but the mechanism of splicing regulation remains poorly understood. In this study, we used a well-investigated alternative splicing reporter to dissect splicing regulatory elements residing in the post-transcriptional reg-ulatory element (PRE) of HBV.
14p cosis54 05-01-2013 57 3 Download
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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Detection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts...
0p thulanh22 20-11-2011 57 2 Download
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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Motif composition, conservation and condition-specificity of single and alternative transcription start sites in the Drosophila genome...
0p thulanh21 12-11-2011 42 1 Download