Balancing oxidation

Balancing oxidation-reduction equations, oxidation-reduction reactions, voltaic cells, cell EMF, effect of concentration on cell EMF is the main content of the lecture "Chapter 20: Electrochemistry". Invite you to consult the detailed content lectures to capture details.

59p leminhuy 01-11-2015 42 4   Download

To establish stable cell lines that produce recombinant multisubunit proteins, it is usually necessary to cotransfect cells with several independent gene constructs. Here, we show that a stepwise fusion of individually transfected cells, results in a fused cell-line that secretes a complete multisubunit protein. Functional expression of recombinant multisubunit proteins may require a defined expression ratio between each protein subunit. The cell-fusion technology described allows a predefined expression level of each subunit.

6p system191 01-06-2013 41 4   Download

Modelling of the fluxes in central metabolism can be performed by combining labelling experiments with metabolite balancing. Using this approach, multiple samples from a cultivation of Saccharomyces cerevisiae in metabolic and isotopic steady state were analysed, and the metabolic fluxes in central metabolism were estimated. In the various samples, the estimates of the central metabolic pathways, the tricarboxylic acid cycle, the oxidative pentose phosphate pathway and the anaplerotic pathway, showed an unprecedented reproducibility....

6p system191 01-06-2013 44 2   Download

The cloned guinea-pig Y2 neuropeptide Y (NPY) receptors expressed in Chinese hamster ovary (CHO) cells, as well as the Y2 receptors natively expressed in rat forebrain, are distributed in two populations. A smaller population that is readily accessed by agonist peptides on the surface of intact cells constitutes less than 30% of Y2 receptors detected in particulates after cell homogenization. A much larger fraction of cell surface Y2 sites can be activated by sulfhydryl modifiers.

8p research12 01-06-2013 43 4   Download

The currently applied reaction structure in stoichiometric flux balance mod-els for the nonoxidative branch of the pentose phosphate pathway is not in accordance with the established ping-pong kinetic mechanism of the enzymes transketolase (EC 2.2.1.1) and transaldolase (EC 2.2.1.2). Based upon the ping-pong mechanism, the traditional reactions of the nonoxida-tive branch of the pentose phosphate pathway are replaced by metabolite specific, reversible, glycolaldehyde moiety (C2 ) and dihydroxyacetone moi-ety (C3) fragments producing and consuming half-reactions. ...

13p fptmusic 12-04-2013 43 3   Download

Both hypoxia and hyperoxia, deregulating the oxidative balance, may play a role in the pathology of neurodegenerative disorders underlain by cerebral ischemia. In the present study, quantitative proton magnetic resonance spectroscopy was used to evaluate regional metabolic alterations, following a 24-hour hypoxic or hyperoxic exposure on the background of ischemic brain insult, in two

9p toshiba23 18-11-2011 50 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Nrf2 protects against pulmonary fibrosis by regulating the lung oxidant level and Th1/Th2 balance...

12p toshiba11 19-10-2011 34 3   Download