Citrate synthase

To examine functions of two small heat shock proteins of Escherichia coli, IbpA and IbpB, we constructed His–IbpA and His–IbpB, in which a polyhistidine tag was fused to the N-terminals. Both purified His–IbpA and His–IbpB formed multimers, which have molecular masses of about 2.0– 3.0 MDa and consist of about 100–150 subunits.

11p system191 01-06-2013 44 4   Download

The crystal structure of citrate synthase from the thermo-philic ArchaeonSulfolobus solfataricus(optimum growth temperature ¼85
C) has been determined, extending the number of crystal structures of citrate synthase from differ-ent organisms to a total of five that span the temperature range over which life exists (from psychrophile to hyper-thermophile). Detailed structural analysis has revealed possible molecular mechanisms that determine the different stabilities of thefiveproteins.

11p tumor12 22-04-2013 63 3   Download

Ở các sinh vật ái khí, pyruvat (tạo thành từ đường phân) bị oxy hóa thành CO2 and acetyl-CoA với sự tham gia của coenzyme A. Sự oxy hóa acetyl –CoA tiếp theo bằng một loạt các phản ứng thuộc chu trình acid citric.

20p m310dy1ndark 21-12-2012 499 54   Download