Genetics System

This paper is aimed to apply hybrid machine learning model namely GA-ANFIS, which is a combination of Adaptive Neuro-Fuzzy Inference System (ANFIS) and Genetic Algorithm (GA), for the prediction of total bearing capability of driven piles.

8p viengfa 28-10-2024 3 2   Download

The research has designed a novel active truck seat suspension system for a further study of active vibration control. A 5-degree-of-freedom driver and seating suspension system model for active vibration control has been developed. A novel fast system parameter identification method from vibration measurement data has been proposed for the seat-occupant system based on the multi-objective Genetic Algorithm optimization (GA).

206p runthenight04 02-02-2023 15 5   Download

To estimate genetic parameters of some growth and reproductive traits, use BLUP method to predicte estimated breeding value (EBV) and selection by EBV to improve the growth performance of pure D, L and Y pigs, improve the reproductive performance of pure L and Y pigs, contribute to meet the requirements of pig production in our country.

27p guitaracoustic09 14-01-2022 18 2   Download

 An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation established an alternative reverse genetics system for influenza virus generation by using an adenovirus vector (AdV)  which achieves highly efficient gene transfer independent of cell transfection efficiency.

4p thuyancn 01-06-2015 33 4   Download

An improved reverse genetics system for influenza A virus generation and its implications for vaccine production present an improved system for the generation of influenza viruses that allows the easy and repro ducible production of vaccine viruses in vero cells. Application of this system may be especially advantageous in situations of outbreaks of highly pathogenic avian influenza viruses.

5p thuyancn 01-06-2015 39 4   Download

Med. Zentrum fur Hygiene und Medizinische Mikrobiologie, Philipps-Universitat Marburg, Marburg, Germany Mannose analogues (2-deoxy-D -glucose, 2-deoxy-2-fluoroD -glucose and 2-amino-2-deoxy-D -mannose) have been used to study glycosylphosphatidylinositol (GPtdIns) biosynthesis and GPtdIns protein anchoring in protozoal and mammalian systems. The effects of these analogues on GPtdIns biosynthesis and GPtdIns-protein anchoring of the human malaria parasite Plasmodium falciparum were evaluated in this study. ...

8p system191 01-06-2013 30 4   Download

We have investigated the crr gene of Streptomyces coelicolor that encodes a homologue of enzyme IIAGlucose of Escherichia coli, which, as a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays a key role in carbon regulation by triggering glucose transport, carbon catabolite repression, and inducer exclusion. As in E. coli, the crr gene of S. coelicolor is genetically associated with the ptsI gene that encodes the general phosphotransferase enzyme I.

8p research12 01-06-2013 42 3   Download

Antizyme is a polyamine-induced cellular protein that binds to ornithine decarboxylase (ODC), and targets it to rapid ubiquitin-independent degradation by the 26S proteasome. However, the metabolic fate of antizyme is not clear. We have tested the stability of antizyme in mammalian cells. In contrast with previous studies demonstrating stability in vitro in a reticulocyte lysate-based degradation system, in cells antizyme is rapidly degraded and this degradation is inhibited by specific proteasome inhibitors. ...

7p research12 01-06-2013 43 3   Download

Binding of lamin B receptor (LBR) to chromatin was studied by means of an in vitro assay system involving recombinant fragments of human LBR and Xenopus sperm chromatin. Glutathione-S-transferase (GST)-fused proteins including LBR fragments containing the N-terminal region (residues 1–53) and arginine-serine repeat-containing region (residues 54–89) bound to chromatin. The binding of GST-fusion proteins incorporating the N-terminal and arginine-serine repeat-containing regions to chromatin was suppressed by mild trypsinization of the chromatin and by pretreatment with a DNA solution....

11p research12 01-06-2013 30 4   Download

All living organisms contain redox systems involving thior-edoxins (Trx), proteins featuringanextremelyconservedand reactive active site that perform thiol-disul®de interchanges with disul®de bridges of target proteins. In photosynthetic organisms, numerous isoforms of Trx coexist, as revealedby sequencing ofArabidopsisgenome. The speci®c functions of many of them are still unknown.

11p research12 29-04-2013 36 2   Download

ThemaltoseATP-binding cassette transporter ofSalmonella typhimurium is composed of a membrane-associated com-plex (MalFGK2) and a periplasmic receptor (MalE). In addition to its role in transport, the complex acts as a repressor ofmaltose-regulatedgene expressionand is subject to inhibition in the process of inducer exclusion. These activities are thought to be mediated by interactions of the ATPase subunit, MalK, with the transcriptional activator, MalT, and nonphosphorylated enzyme IIA of the glucose phosphotransferase system, respectively. ...

12p research12 23-04-2013 37 3   Download

Serotonin transporters are key target sites for clinical drugs and psychostimulants, such as fluoxetine and cocaine. Molecular cloning of a serotonin transporter from the cen-tral nervous system of the insectManduca sextaenabled us to define domains that affect antagonist action, particularly cocaine.

11p research12 23-04-2013 37 2   Download

Acollagen-likeproteinwas identi®ed fromtheotolithsof the chum salmon,Oncorhynchus keta. The otolith, composed mainly of calcium carbonate with small amount of organic matrices, is formed in the inner ear and serves as apart of the hearing and balance systems. Although the organicmatrices may play important roles in the growth of otolith, little is known about their chemical nature and physiological func-tion. In this study, amajor organic component of theotolith, designated otolin-1, which may serve as a template for calci®cation, was puri®ed. ...

9p research12 23-04-2013 33 2   Download

Asheat shockproteins (Hsps) are involved inprotecting cells and also in the pathophysiology of diseases such as inflam-mation, cancer and neurodegenerative disorders, modula-tors ofHsp expression inmammalian cells would seemto be useful for the treatment of various diseases. In this study, we isolated mammalian cell lines for screening of Hsp modu-lators; mouse C3H10T1/2 cells stably transfected with a plasmid containing the mouse Hsp105 or human Hsp70B promoter upstream of a luciferase or b-galactosidase reporter gene, respectively....

8p tumor12 20-04-2013 37 5   Download

The ethanol utilization pathway inAspergillus nidulansis a model system, which has been thoroughly elucidated at the biochemical, genetic and molecular levels. Three main ele-ments are involved: (a) high level expression of the positively autoregulated activator AlcR; (b) the strong promoters of the structural genes for alcohol dehydrogenase (alcA)and aldehyde dehydrogenase (aldA); and (c) powerful activa-tion of AlcRby the physiological inducer, acetaldehyde, produced from growth substrates such as ethanol and L-threonine. ...

10p tumor12 20-04-2013 24 2   Download

The cell line, RCS-LTC (derived from the Swarm rat chondrosarcoma), deposits a copious extracellular matrix in which the collagen component is primarily a polymer of partially processed type II N-procollagen molecules. Transmission electron microscopy of the matrix shows no obvious fibrils, only a mass of thin unbanded filaments. We have used this cell system to show that the type II N-pro-collagen polymer nevertheless is stabilized by pyridinoline cross-links at molecular sites (mediated by N- and C-telo-peptide domains) found in collagen II fibrils processed nor-mally....

8p tumor12 20-04-2013 35 2   Download

It is known that phosphatidylinositol (PtdIns) contains abundant arachidonate and is composed mainly of 1-stea-royl-2-arachidonoyl species in mammals. We investigated if this characteristic of PtdIns applies to the PtdIns from yellowtail (Seriolaquinqueradiata),amarinefish. Incommon with phosphatidylcholine (PtdCho),phosphatidylethanol-amine (PtdEtn) andphosphatidylserine (PtdSer) frombrain, heart,liver,spleen,kidney and ovary,the predominant polyunsaturated fatty acid was docosahexaenoic acid,and levels of arachidonic acid were less than 4.5% (PtdCho), 7.5%(PtdEtn) and3.

8p tumor12 20-04-2013 41 3   Download

Targeting of proteins to and translocation across the membranes is a fundamental biological process in all organisms. In bacteria, the twin arginine translocation (Tat) system can transport folded proteins. Here, we demonstrate in vivo that the high potential iron-sulfur protein (HiPIP) from Allochromatium vinosumis trans-located into the periplasmic space by the Tat system of Escherichia coli. In vitro, reconstituted HiPIP precursor (preHoloHiPIP) was targeted to inverted membrane vesicles from E. ...

11p tumor12 20-04-2013 35 2   Download

Spatial organizationofwild-type (strainU1) tobaccomosaic virus (TMV) and of the temperature-sensitive TMV ts21–66 mutant was compared by tritium planigraphy. The ts21–66 mutant contains two substitutions in the coat protein (Ile21fiThr and Asp66fiGly) and, in contrast withU1, induces a hypersensitive response (formation of necroses) on the leaves of plants bearing a host resistance geneN¢(for example Nicotiana sylvestris); TMV U1 induces systemic infection (mosaic) on the leaves of such plants.

9p fptmusic 16-04-2013 53 4   Download

The results of the present study show that human platelets take up L-arginine by two transport systems which are compatible with the systems y + and y + L. These Na + -independent transporters have been distinguished by treat-ing platelets with N-ethylmaleimide that blocks selectively systemy + .Systemy + , that accounts for 30–40%of the total transport, is characterized by low affinity forL-arginine, is unaffected byL-leucine, is sensitive to changes of membrane potential and totrans-stimulation. ...

8p fptmusic 16-04-2013 41 3   Download