Hepatitis C

Chronic liver disease has a high global burden and death toll. Early diagnosis is important to halt disease progression to cirrhosis, hepatocellular carcinoma, and eventually death. At present, there are many methods used for liver disease assessment, such as liver biopsy, elastography, serum biomarkers, and surrogate markers.

5p vihizuzen 26-05-2025 3 1   Download

Hepatitis A là một trong 5 loại virus truyền nhiểm gây ra bệnh viêm gan cho người, đó là Hepatitis A, B, C, D và E. Loại virus này hiếm thấy ở những nước có hệ thống vệ sinh công cộng tốt như ở Mỹ. Tuy vậy hàng năm ước tính có khoảng 100 người chết do viêm gan có liên quan đến virus Hepatitis A (theo tài liệu CDC1) trong số 30 – 50.000 ca bệnh, hao tốn trên 300 triệu USD

80p lalan38 29-03-2013 125 19   Download

This research aiming to: Describing clinical and characteristics laboratory features and identifying HCV genotype on patients of HCC. Evaluating relationship between HCV genotypes and some clinical characteristics laboratory features,on patients of HCC.

27p slimzslimz 21-12-2019 33 9   Download

Schistosomiasis is an endemic disease in Egypt caused by the trematode Schistosoma which has different species. c the best known form of chronic disease with a wide range of clinical manifestations. The pathogenesis of schistosomiasis is related to the host cellular immune response. This leads to granuloma formation and neo angiogenesis with subsequent periportal fibrosis manifested as portal hypertension, splenomegaly and esophageal varices. Intestinal schistosomiasis is another well identified form of chronic schistosomal affection.

8p kethamoi1 17-11-2019 31 2   Download

Glycolysis from [6-3 H]glucose and gluconeogenesis from [U-14 C]glycerol were examined in isolated hepatocytes from fasted rats. A 5 mMbolus of glycerol inhibited phosphory-lationof 40 mMglucoseby50%andglycolysis bymore than 60%, and caused cellular ATP depletion and glycerol 3-phosphate accumulation. Gluconeogenesis from 5 mM glycerol was una€ected by the presence of 40 mMglucose.

6p research12 01-06-2013 51 6   Download

Fructose 2,6-bisphosphate (Fru-2,6-P2 ) plays an important role in the regulation of major carbohydrate fluxes as both allosteric activator and inhibitor of target enzymes. To examine the role of Fru-2,6-P2in the regulation of hepatic carbohydrate metabolism in vivo,Fru-2,6-P2 levels were elevated in ADM mice with adenovirus-mediated overex-pression of a double mutant bifunctional enzyme, 6-phos-phofructo-2-kinase/fructose-2,6-bisphosphatase (n¼6), in comparison to normal control mice (control,n¼6). ...

9p research12 29-04-2013 51 5   Download

Human hepatitis delta (HDV) ribozyme is one of small ribozymes, such as hammerhead and hairpin ribozymes, etc. Its secondary structure shows pseudoknot structure com-posed of four stems (I to IV) and three single-stranded regions (SSrA, -B and -C). The 3D structure of 3¢-cleaved product of genomic HDV ribozyme provided extensive information about tertiary hydrogen bonding interactions between nucleotide bases, phosphate oxygens and 2¢OHs including new stem structure P1.1.

12p tumor12 22-04-2013 40 3   Download

A search has been initiated for lead inhibitors of the non-structural protein 3 (NS3)-associated NTPase/helicase activities of hepatitis C virus,the related West Nile virus, Japanese encephalitis virus and the human mitochondrial Suv3 enzyme. Random screening of a broad range of unre-lated low-molecular mass compounds,employing both RNA and DNA substrates, revealed that 4,5,6,7-tetra-bromobenzotriazole (TBBT) hitherto known as a potent highly selective inhibitor of protein kinase 2,is a good inhibitor of the helicase,but not NTPase,activity of hepa-titis C virus NTPase/helicase. ...

9p fptmusic 16-04-2013 43 4   Download

In our attempt to obtain further information on the replication mechanism of the hepatitis C virus (HCV), we have studied the role of sequences at the 3¢-end of HCV minus-strand RNA in the initiation of synthesis of the viral genome by viral RNA-dependent RNA polymerase (RdRp). In this report, we investigated the template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in the presence of viral polymerase.

15p fptmusic 11-04-2013 55 3   Download

A bio-nanocapsule (BNC), composed of the surface antigen (sAg) of the hepatitis B virus, is an efficient nanomachine with which to accomplish the liver-specific delivery of genes and drugs. Approximately 110 molecules of sAg are associated to form a BNC particle with an average diameter of 130 nm. TheLprotein is an sAg peptide composed mainly of preS and S regions. The preS region, with specific affinity for human hepatocytes, is localized in the N-terminus.

10p fptmusic 11-04-2013 41 2   Download

The interaction with phospholipid bilayers of two synthetic peptides with sequences corresponding to a segment next to the native N-terminus and an internal region of the E2 structural hepatitis G virus (HGV⁄GBV-C) protein [E2(7–26) and E2(279–298), respectively] has been characterized. Both peptides are water soluble but associate spontaneously with bilayers, showing higher affinity for anionic than zwitterionic membranes.

11p awards 06-04-2013 39 3   Download

Chronic hepatitis C virus (HCV) infection is a clinically important liver disease with limited therapeutic options in a significant proportion of patients. Therefore, novel efficient therapeutic agents are needed. Because the 5¢- and 3¢-untranslated regions (UTRs) of HCV are highly conserved and functionally important for HCV replication, they are attractive targets for RNA-cleaving ribozymes or small interfering RNAs (siRNAs).

13p dell39 27-03-2013 52 5   Download

Hepatitis C virus (HCV) exists in different forms in the circulation of infec-ted people: lipoprotein bound and lipoprotein free, enveloped and non-enveloped. Viral particles with the highest infectivity are associated with lipoproteins, whereas lipoprotein-free virions are poorly infectious.

15p inspiron33 25-03-2013 34 4   Download

Translating proteases as inactive precursors, or zymogens, protects cells from the potentially lethal action of unregulated proteolytic activity. Here, we impose this strategy on bovine pancreatic ribonuclease (RNase A) by creating a zymogen in which quiescent ribonucleolytic activity is activated by the NS3 protease of the hepatitis C virus.

9p inspiron33 23-03-2013 37 4   Download

Using thein situ liver model system, we have recently shown that, after cholera toxin binding to hepatic cells, cholera toxin accumulates in a low-density endosomal compartment, and then undergoes endosomal proteoly-sis by the aspartic acid protease cathepsin-D [Merlen C, Fayol-Messaoudi D, Fabrega S, El Hage T, Servin A, Authier F (2005)FEBS J272, 4385– 4397]. Here, we have used a subcellular fractionation approach to address the in vivo compartmentalization and cytotoxic action of cholera toxin in rat liver parenchyma....

16p galaxyss3 21-03-2013 58 4   Download

Translation initiation of hepatitis C virus (HCV) occurs through an internal ribosome entry site (IRES) located at its 5¢-end. As a positive-stranded RNA virus, HCV uses its genome as a common template for translation and replication, but the coordination between these two pro-cesses remains poorly characterized.

19p galaxyss3 07-03-2013 36 5   Download

This study of the full-length bifunctional nonstructural protein 3 from hep-atitis C virus (HCV) has revealed that residues in the helicase domain affect the inhibition of the protease. Two residues (Q526 and H528), appar-ently located in the interface between the S2 and S4 binding pockets of the substrate binding site of the protease, were selected for modification, and three enzyme variants (Q526A, H528A and H528S) were expressed, puri-fied and characterized.

8p media19 05-03-2013 35 4   Download

Hepatitis C virus is a human pathogen responsible for liver diseases includ-ing acute and chronic hepatitis, cirrhosis and hepatocellular carcinoma. Its high prevalence, the absence of a prophylactic vaccine and the poor effi-ciency of current therapies are huge medical problems. Since the discovery of the hepatitis C virus, our knowledge of its biology has been largely punctuated by the development of original models of research.

14p media19 04-03-2013 35 3   Download

Toll-like receptors and RNA helicase family members [retinoic acid-inducible gene I (RIG-I) and melanoma differentiation associated gene-5 (MDA5)] play important roles in the induction of interferon-bas a major event in innate immune responses after virus infection. TRIF (adaptor pro-tein of Toll-like receptor 3)-mediated and Cardif (adaptor protein of RIG-I or MDA5)-mediated signaling pathways contribute rapid induction of interferon-bthrough the activation of interferon regulatory factor-3 (IRF-3)....

16p media19 04-03-2013 39 3   Download

Recent studies have suggested the existence of a novel protein of hepati-tis C virus (HCV) encoded by an ORF overlapping the core gene in the +1 frame (core+1 ORF). Two alternative translation mechanisms have been proposed for expression of the core+1 ORF of HCV-1a in cultured cells; a frameshift mechanism within codons 8–11, yielding a protein known as core+1⁄F, and⁄or translation initiation from internal codons in the core+1 ORF, yielding a shorter protein known as core+1⁄S.

18p media19 04-03-2013 38 3   Download