Knowledge transfer

This study investigates the interaction between knowledge transfer, change management, and leadership in promoting innovation within the digital education ecosystem of Vietnamese universities.

12p viling 11-10-2024 1 0   Download

This research article explores the potential of active learning pedagogies to enhance student engagement, knowledge retention, and essential skill development in higher education. These learner-centered approaches, such as experiential, project-based, and inquiry-based learning, contrast with traditional lecture-based methods that prioritize passive information transfer.

7p vipanly 28-10-2024 2 1   Download

Organizations have long struggled with the successful completion of projects within the time, cost and performance indicators with respect to a customer product or solution that is expected to ultimately meet the customer’s expectations. This thesis demonstrates the link between the successful creation, management and dissemination of project requirements throughout the entire project process using a knowledge management overlay model to ensure the success of the overall project to meet stakeholder needs through the successful creation and transfer of project requirements.

353p runthenight04 02-02-2023 12 4   Download

he outcome of projects as TALISMAN (I-3), EFNUDAT/NUDAME (I-3), GENTLE (mobility), ENEN-plus (mobility), NUGENIA-plus (mobility within TA of NUGENIA) and ESNII-plus (I-3 similar) will be shortly presented as well as the future European Commission plans in the field of access to research infrastructure.

7p christabelhuynh 29-05-2020 10 1   Download

Many biological C-H activation reactions exhibit nonclassical kinetic isotope effects (KIEs). These nonclassical KIEs are too large (kH/kD 7) and/or exhibit unusual temperature dependence such that the Arrhenius prefactor KIEs (AH/AD) fall outside of the semiclassical range near unity. The focus of this minireview is to discuss such KIEs within the context of the environmentally coupled hydrogen tunneling model.

9p system191 01-06-2013 41 4   Download

Mucopolysaccharidosis type I (MPS I) results from a deficiency in the enzyme a-L-iduronidase (IDUA), and is characterized by skeletal abnormalities, hepatosplenomegaly and neurological dysfunction. In this study, we used a late generation lentiviral vector to evaluate the utility of this vector system for the transfer and expression of the human IDUA cDNA in MPS I fibroblasts. We observed that the level of enzyme expression in transduced cells was 1.5-fold the level found in normal cells; the expression persisted for at least two months....

8p system191 01-06-2013 36 5   Download

The ¯uorescence resonance energy transfer parameter, f, is de®ned as the eciency of the energy transfer normalized by the quantum yield of the donor in the presence of acceptor. It is possible to characterize the ¯exibility of the protein matrix between the appropriate ¯uorescent probes by monitoring the temperature dependence of f. The intermonomer ¯exibility of the Ca-actin and Mg-actin ®laments was characterized by using this method at pH values of 6.5 and 7.4.

8p research12 01-06-2013 59 4   Download

Special oligonucleotides for targeted gene correction have attracted increasing attention recently, one of which is the chimericRNAÆDNAoligonucleotide (RDO) system. RDOs for targeted gene correction were first designed in 1996, and are typically 68 nucleotides in length including continuous RNA andDNA sequences (RNA is 2¢-O-methyl-modified).

6p tumor12 22-04-2013 36 2   Download

The opportunity to design enzymatic systems is becoming more feasible due to detailed knowledge of the structure of many proteins. As a first step, investigations have aimed to redesign already existing systems, so that they can performa function different from the one for which they were syn-thesized. We have investigated the interaction of electron transfer proteins fromdifferent systems inorder to check the possibility of heterologous reconstitution among members of different chains.

10p tumor12 20-04-2013 39 3   Download

The role of coenzyme binding in regulating interflavin electron transfer in human cytochrome P450 reductase (CPR) has been studied using temperature-jump spectros-copy. Previous studies [Gutierrez, A., Paine, M., Wolf, C.R., Scrutton, N.S., & Roberts, G.C.K.Biochemistry (2002) 41,4626–4637] have shown that the observed rate, 1/s, of interflavin electron transfer (FADsq )FMNsq fi FADox)FMNhq) inCPRreduced at the two-electron level with NADPH is 55 ± 2 s )1 , whereas with dithionite-reduced enzyme the observed rate is 11 ± 0.

10p fptmusic 16-04-2013 36 4   Download

The roles of His181, His184 and Tyr186 in PETN reductase have been examined by mutagenesis, spectroscopic and stopped-flow kinetics, and by determination of crystallographic structures for the Y186F PETN reductase and reduced wild-type enzyme—progesterone complex. Residues His181 and His184 are important in the binding of coenzyme, steroids, nitro-aromatic ligands and the substrate 2-cyclohexen-1-one.

12p fptmusic 12-04-2013 43 3   Download

Ultrasensitive responses are common in cellular information transfer because they allow cells to decode extracellular stimuli in an all-or-none manner. Biochemical responses are usually analyzed by fitting the Hill equation, and the estimated Hill coefficient is taken as a measure of sensi-tivity. However, this approach is not appropriate if the response under con-sideration significantly deviates from the best-fit Hill equation.

9p fptmusic 11-04-2013 33 2   Download

A wide range of approaches has been applied to examine the quaternary structure of G protein-coupled receptors, the basis of such protein–protein interactions and how such interactions might modulate the pharmacology and function of these receptors. These include coimmunoprecipitation, var-ious adaptations of resonance energy transfer techniques, functional com-plementation studies and the analysis of ligand-binding data.

12p fptmusic 11-04-2013 52 3   Download

Transcription of yeast phospholipid biosynthesis structural genes, which contain an inositol-sensitive upstream activa-ting sequence in their promoters, responds to the availability of the soluble precursors inositol and choline and to changes in phospholipid metabolism.

8p awards 05-04-2013 41 3   Download

3,3¢-Dipropyl-2,2¢-thiadicarbocyanine iodide [DiS-C3 (5)], often used as a tracer dye to assess the mitochondrial mem-brane potential, was investigated in detail regarding its effects on the structure and function of isolated mitochon-dria. As reported previously, DiS-C3(5) had an inhibitory effect on NADH-driven mitochondrial electron transfer. On the contrary, in the presence of inorganic phosphate, DiS-C3(5) showed dose-dependent biphasic effects on mito-chondria energized by succinate. At higher concentrations, such as 50lM,DiS-C3(5) acceleratedmitochondrial oxygen consumption. ...

0p awards 05-04-2013 39 2   Download

To test thioredoxin resistance to oxidizing free radicals, we have studied the one-electron oxidation of wild-type thio-redoxin and of two forms with the point mutations D30A and W35A, using azide radicals generated byc-ray or pulse radiolysis. The oxidation patterns of wild-type thioredoxin and D30A are similar. In these forms, Trp35 is the primary target and is
repaired by one-electron reduction; first by intramolecular electron transfer from tyrosine, and then fromother residues. Conversely, duringoxidationofW35A, Trp13 is poorly reactive.

7p awards 05-04-2013 40 4   Download

Several ribonucleases, including onconase anda-sarcin, are known to be toxic to tumor cells. On the other hand, although its structure is relatedto thatofa-sarcin,RNase T1 is noncytotoxic because of its inability to internalize into tumor cells. In this study, we internalized RNase T1 into human tumor cells via a novel gene transfer reagent, hem-agglutinating virus of Japan (HVJ) envelope vector, which resulted in cell death.

6p dell39 03-04-2013 39 5   Download

Horizontal gene transfer has been a major force for genome plasticity over evolutionary history, and is largely responsible for fitness-enhancing traits, including antibiotic resistance and virulence factors. In particular, for adap-tation of prokaryotes to extreme environments, lateral gene transfer seems to have played a crucial role.

9p inspiron33 25-03-2013 43 4   Download

Sterol carrier protein-2 (SCP-2) is a small intracellular basic protein domain implicated in peroxisomalb-oxidation. We extend our knowledge of plant SCP-2 by characterizing SCP-2 fromEuphorbia lagascae. This pro-tein consists of 122 amino acids including a PTS1 peroxisomal targeting signal.

15p inspiron33 23-03-2013 45 3   Download

This study on human cytochrome P450 reductase (CPR) presents a com-prehensive analysis of the thermodynamic and kinetic effects of pH and solvent on two- and four-electron reduction in this diflavin enzyme. pH-dependent redox potentiometry revealed that the thermodynamic equilibrium between various two-electron reduced enzyme species (FMNH • ,FADH • ; FMN,FADH2; FMNH2 ,FAD) is independent of pH.

18p galaxyss3 07-03-2013 46 3   Download