Metalloprotease

Matrilysin (matrix metalloproteinase-7) plays important roles in tumor progression. It was previously found that matrilysin binds to the surface of colon cancer cells to promote their metastatic potential. In this study, we identified annexin II as a novel membrane-bound substrate of matrilysin. Treatment of human colon cancer cell lines with active matrilysin released a 35 kDa annexin II form, which lacked its N-terminal region, into the culture supernatant. The release of the 35 kDa annexin II by matrilysin was significantly enhanced in the presence of serotonin or heparin....

14p research12 29-04-2013 42 3   Download

Mortality in the host armyworm larvaePseudaletia separata parasitized by the parasitic waspCotesia kariyaiwas dra-matically increased when they were simultaneously infected by the entomopathogen Serratia marcescens. Previous studies have shown that this strong insecticidal effect is due to a metalloprotease-like insecticide (MPLI)released from S. marcescensenterobacter. This study was conducted to elucidate the exact cause of the mortality resulting from MPLI.

8p tumor12 20-04-2013 42 2   Download

Streptomyces mobaraensis secretes a Ca 2+ -independent transglutaminase (TGase) that is activated by removing an N-terminal peptide from a precursor protein during sub-merged culture in a complex medium [Pasternack, R., Dorsch, S., Otterbach, J. T., Robenek, I. R., Wolf, S. & Fuchsbauer, H.-L. (1998)Eur. J. Biochem. 257, 570–576]. However, an activating protease could not be identified, probably because of the presence of a 14-kDa protein (P14 ) belonging to theStreptomycessubtilisin inhibitor family. ...

9p tumor12 20-04-2013 33 3   Download

In the nematodeCaenorhabditis elegans, 40 genes code for astacin-likeproteins (nematodeastacins,NAS).The astacins are metalloproteases present in bacteria, invertebrates and vertebrates and serve avarietyof physiological functions like digestion, hatching, peptide processing, morphogenesis and pattern formation. With the exception of one distorted pseudogene, all the otherC. elegansastacins are expressed and are evidently functional. For 13 genes we found splicing patterns differing from the Genefinder predictions in WormBase, sometimes markedly....

12p fptmusic 12-04-2013 39 3   Download

Numerous transmembrane proteins, including the blood pressure regulating angiotensin converting enzyme (ACE) and the Alzheimer’s disease amyloid precursor protein (APP), are proteolytically shed from the plasma membrane by metalloproteases. We have used an antisense oligo-nucleotide (ASO) approach to delineate the role of ADAM10 and tumour necrosis factor-aconverting enzyme (TACE;ADAM17) in theectodomainsheddingofACEand APP from human SH-SY5Y cells.

9p dell39 03-04-2013 57 3   Download

The CIII protein of bacteriophage lambda exhibits antiproteolytic activity against the ubiquitous metalloprotease HflB (FtsH) of Escherichia coli, thereby stabilizing the kCII protein and promoting lysogenic development of the phage. CIII also protectsE. colir 32 , another substrate of HflB.

6p inspiron33 23-03-2013 34 4   Download

The activation of ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs) family proteases depends on removal of the prodomain. Although several studies suggest that ADAMTS activities play roles in development, homeostasis and disease, it remains unclear when and where the enzymes are activated in vivo.

10p galaxyss3 07-03-2013 37 5   Download

TheVibriometalloprotease PrtV was purified from the culture supernatant of aVibrio choleraederivative that is deficient in several other secreted pep-tidases, including the otherwise abundant hemagglutinin⁄protease HapA. The PrtV is synthesized as a 102 kDa protein, but undergoes several N- and C-terminal processing steps duringV. choleraeenvelope transloca-tion and prolonged incubation.

11p galaxyss3 07-03-2013 44 3   Download

The hatching enzyme of oviparous euteleostean fishes consists of two metalloproteases: high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE). They cooperatively digest the egg envelope (chorion) at the time of embryo hatching. In the present study, we investigated the hatching of embryos of the ovoviviparous black rockfish Sebastes schlegelii.

15p galaxyss3 07-03-2013 40 3   Download

Ticks are obligate blood-feeding arachnids. During their long-lasting blood meal, they have to counteract the protective barriers and defense mecha-nisms of their host. These include tissue integrity, pain, hemostasis, and the inflammatory and immune reactions. Here, we describe a multigene family coding for five putative salivary metalloproteases induced during the blood meal ofIxodes ricinus.

15p media19 06-03-2013 50 3   Download

Vibrio mimicusis a causative agent of human gastroenteritis and food poisoning, and this species produces an enterotoxic hemolysin (V. mimi-cus hemolysin) as a virulence determinant. Vibrio mimicushemolysin is secreted as an 80 kDa precursor, which is later converted to the 66 kDa mature toxin through removal of an N-terminal propeptide via cleavage of the Arg151–Ser152 bond.

10p vinaphone15 27-02-2013 37 4   Download

Matrix metalloprotease-2 is implicated in many biological processes and degrades extracellular and non-extracellular matrix molecules. Matrix metalloprotease-2 maintains a latent state through a cysteine–zinc ion pairing which, when disrupted, results in full enzyme activation. This pairing can be disrupted by a conformational change or cleavage within the propeptide.

14p viettel02 20-02-2013 50 3   Download

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a proteinase involved in the remodelling of extracellular matrix and the cleavage of a number of substrates. MT1-MMP is synthesized as a zymogen that requires intracellular post-translational cleavage to gain biological activity. Furin, a member of the pro-protein convertase family, has been implicated in the proteolytic removal of the MT1-MMP prodomain sequence.

18p viettel02 19-02-2013 35 4   Download

Homologous bacterial metalloproteases ECP32⁄grimelysin from Serratia grimesiiand protealysin fromSerratia proteamaculansare involved in the invasion of the nonpathogenic bacteria in eukaryotic cells and are sug-gested to translocate into the cytoplasm [Bozhokina ESet al. (2011) Cell Biol Int35, 111–118].

11p cosis54 08-12-2012 31 3   Download

Liver fibrosis ranks as the second cause of death in México's productive-age population. This pathology is characterized by acummulation of fibrillar proteins in hepatic parenchyma causing synthetic and metabolic disfunction. Remotion of excessive fibrous proteins might result in benefit for subjects increasing survival index. The goal of this work was to find whether the already known therapeutical effect of human urokinase Plasminogen Activator and human Matrix Metalloprotease 8 extends survival index in cirrhotic animals.

9p toshiba23 18-11-2011 51 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" The role of polymorphisms in ADAM33, a disintegrin and metalloprotease 33, in childhood asthma and lung function in two German populations...

12p toshiba13 21-10-2011 51 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expression of the metalloproteases MMP-1, MMP-2, MMP-3, MMP-9, MMP-11, TIMP-1 and TIMP-2 in angiocentric midfacial lymphomas

9p matuot_238 13-10-2011 74 2   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13: identification of a new biglycan cleavage site...

9p thulanh12 13-10-2011 32 2   Download