Molecular diagnostics

Tuberculosis (TB) remains a major global health challenge, with early and accurate diagnosis being critical for effective disease control. Traditional acid-fast bacilli (AFB) smear microscopy has limited sensitivity, particularly in smear-negative cases. The advent of molecular techniques, such as the GeneXpert MTB/RIF assay and real-time PCR, has significantly improved the rapid detection of Mycobacterium tuberculosis (MTB) and rifampicin resistance.

6p vihizuzen 26-05-2025 1 1   Download

The current research project genotyped HAV RNA by using protocols and primer sets designed by HAVNET. It collected and genotyped previous HAV-positive samples in VIDRL sample bank, and the results were used to establish epidemiological and molecular database of the HAV strains. The database was used to investigate the relationship between the current diagnostic samples and the previous HAV infection cases. It was expected to identify the source of the hepatitis A infection.

158p runthenight04 02-02-2023 13 3   Download

Diagnostic tests by molecular biology is made for studying the relations among Fusarium species for linking production of proteins, degree of relationship and occurrence of malformation. Determination of proteins for isolates causing-disease by SDS-PAGE explained there’s specific band for each fungus and there are common bands among some isolates of fungi. Since, band with MW 30 KDa represented only in F. proliferatum and F. oxysporum and F. subgluti-nans respectively. This band considered as specific band for these isolates, which released high patho-genisity effect.

7p zingzing09 24-04-2013 60 3   Download

Reliable detection and identification of pathogens in complex biological samples, in the presence of contaminating DNA from a variety of sources, is an important and challenging diagnostic problem for the development of field tests. The problem is compounded by the difficulty of finding a single, unique genomic sequence that is present simultaneously in all genomes of a species of closely related pathogens and absent in the genomes of the host or the organisms that contribute to the sample background. ...

11p dell39 27-03-2013 37 4   Download

a-enolase (ENOA) is a metabolic enzyme involved in the synthesis of pyru-vate. It also acts as a plasminogen receptor and thus mediates activation of plasmin and extracellular matrix degradation. In tumor cells, EMOAis upregulated and supports anaerobic proliferation (Warburg effect), it is expressed at the cell surface, where it promotes cancer invasion

0p cosis54 05-01-2013 47 3   Download

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer

13p dauphong3 03-01-2012 51 3   Download

Aleksandra Klemba1,2, Magdalena Kowalewska3, Wojciech Kukwa4, Katarzyna Tonska1, Aleksandra Szybinska5, Malgorzata Mossakowska5, Anna Scinska4, Paweł Golik1,6, Kamil Koper1, Jakub Radziszewski7, Andrzej Kukwa4, Anna M Czarnecka1,2*, Ewa Bartnik1,6 Abstract Vulvar squamous cell carcinoma (VSCC) is a rare female genital neoplasm. Although numerous molecular changes have been reported in VSCC, biomarkers of clinical relevance are still lacking. On the other hand, there is emerging evidence on the use of mtDNA as a diagnostic tool in oncology.

15p toshiba23 18-11-2011 58 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The clinical utility of molecular diagnostic testing for primary immune deficiency disorders: a case based review...

9p thulanh10 09-10-2011 61 1   Download