The shift of structure

The rapid development of manufacturing sectors in the contemporary Industry 4.0 era has prompted enterprises to adopt increasingly systematic, streamlined, and efficient organizational structures. In this scenario, production scheduling has arisen as an essential function that contributes to the effective allocation of organizational resources in the production process, while shortening production time and satisfying customer deadlines.

6p vibenya 31-12-2024 4 2   Download

Analyze real situation of shifting the structure of economics during the process of industrialization, modernization in Mekong Delta for the stage of 2000-2017. On that basis, assess the achievement, limit and define essential reasons of limitations during the process of shifting the structure of economics in the zone for this stage.

30p dungmaithuy 17-09-2019 43 5   Download

P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. When the UIC2-shift was elicited by certain modulators (e.g. cyclosporin A or vinblastine, but not with verapamil or Tween 80), the subsequent binding of other monoclonal anti-Pgp Ig sharing epitopes with UIC2 (e.g. MM12.10) was abolished [Nagy, H., Goda, K., Arceci, R., Cianfriglia, M., ´ Mechetner, E. & Szabo Jr, G.

6p system191 01-06-2013 63 7   Download

Unfolding, inactivation and dissociation of the lectin from Artocarpus hirsutaseedswere studiedbychemical (guanidine hydrochloride, GdnHCl) and thermal denaturation. Con-formational transitions were monitored by intrinsic ¯uor-escence and circular dichroism. The gradual red shift in the emissionmaxima of the native protein from335 to 356 nm, change intheellipticityat 218 nmandsimultaneousdecrease in the sugar binding activity were observed with increasing concentration of GdnHCl in the pH range between 4.0 and 9.0. ...

5p research12 29-04-2013 47 3   Download

Achromobacterprotease I (API) has a unique region of aromatic ring stacking with Trp169–His210in close proxi-mity to the catalytic triad. This paper reveals the electrostatic role of aromatic stacking in the shift in optimum pH to the alkaline region, which is the highest pH range (8.5–10) among chymotrypsin-type serine proteases. The pH-activity profile of API showed a sigmoidal distribution that appears at pH 8–10, with a shoulder at pH 6–8.

7p research12 23-04-2013 43 3   Download

Correct sorting of newly synthesized peroxisomal matrix proteins is dependent on a peroxisomal targeting signal (PTS).So far two PTSs are known.PTS1 consists of a tripeptide that is located at the extremeCterminus ofmatrix proteins and is specifically recognized by the PTS1-receptor Pex5p.We studied Hansenula polymorpha Pex5p (HpPex5p) using fluorescence spectroscopy.The intensity of Trp fluorescence of purified HpPex5p increased by 25% upon shifting the pH frompH 6.0 to pH 7.2.

7p tumor12 20-04-2013 42 4   Download

The structural changes produced by the minor-groove binding ligand DAPI (4¢,6-diamidine-2-phenylindole) on individual strands of trinucleotide repeat sequences were detectedby electrophoreticband-shift analysis andrelated to their effects on DNA replication in vitro. Among the 20 possible single-stranded trinucleotide repeats, only theT-rich strand of the AATÆATT triplet exhibits an observable fluorescence band and a change in electrophoretic mobility due to the drug binding.

7p fptmusic 12-04-2013 46 4   Download

The fermentation ofb-alanine by Clostridium propionicumproceeds via activation to the CoA-thiol ester, followed by deamination to acryloyl-CoA, which is also an intermediate in the fermentation ofl-alanine. By shifting the organism from the carbon and energy source a-alanine to b-alanine, the enzyme b-alanyl-CoA:ammonia lyase is induced 300-fold (30% of the soluble protein). The low basal lyase activity is encoded by theacl1gene, whereas the almost identicalacl2gene (six amino acid substi-tutions) is responsible for the high activity after growth on b-alanine....

0p awards 05-04-2013 46 3   Download

A highly purified cytochromeb6f complex from the cyanobacterium Syn-echocystissp. PCC 6803 selectively binds one chlorophyll a and one caro-tenoid in analogy to the recent published structure from two other b6 f complexes. The unknown function of these pigments was elucidated by spectroscopy and site-directed mutagenesis. Low-temperature redox differ-ence spectroscopy showed red shifts in the chlorophyll and carotenoid spec-tra upon reduction of cytochrome b6, which indicates coupling of these pigments with the heme groups and thereby with the electron transport....

11p awards 05-04-2013 40 3   Download

The NMR solution structures of NTX-1 (PDB code 1W6B and BMRB 6288), a long neurotoxin isolated from the venom ofNaja naja oxiana, and the molecular dynamics simulation of these structures are reported. Calculations are based on 1114NOEs, 19 hydrogen bonds, 19 dihedral angle restraints and secondary chemical shifts derived from 1 Hto 13 C HSQC spectrum. Similar to other long neurotoxins, the three-finger like structure shows a double and a triple strandedb-sheet aswell as some flexible regions, particularly at the tip of loop II and the C-terminal tail....

0p awards 05-04-2013 50 2   Download

Membrane-associated calcium-independent phospholipase A2c(iPLA2c) contains four potential in-frame methionine start sites (Mancuso, D.J. Jenkins, C.M. & Gross, R.W. (2000)J. Biol. Chem.275, 9937–9945), but the mechanisms regulating the types, amount and subcellular localization of iPLA2c in cells are incompletely understood.

16p awards 05-04-2013 58 3   Download

The effect ofb-lactoglobulin encapsulation in sodium bis (2-ethylhexyl) sulfosuccinate reverse micelles on the envi-ronment of protein and on Trp was analysed at different water contents (x0).CDdataunderlined thedistortionof the b-sheet and a less constrained tertiary structure as the x0 increased, in agreement with a concomitant red shift and a decrease in the signal intensity obtained in steady-state fluorescence measurements.

11p dell39 03-04-2013 43 3   Download

The methanogenic archaeaMethanococcus jannaschiiand M. maripaludiscontain an atypical seryl-tRNA synthetase (SerRS), which recognizes eukaryotic and bacterial tRNAs Ser , in addition to the homologous tRNA Ser and tRNA Sec species.The relativeflexibility in tRNArecognition displayed by methanogenic SerRSs, shown by aminoacyla-tion and gel mobility shift assays, indicates the conservation of some serine determinants in all three domains. The complex of M. maripaludisSerRS with the homologues tRNA Ser was isolated by gel filtration chromatography....

9p dell39 03-04-2013 45 5   Download

The effects of high hydrostatic pressure (HHP) and urea on conformational transitions of humana-thrombin structure were studiedbyfluorescence spectroscopy andbymeasuring the catalytic activity of the enzyme. Treatment of thrombin with urea produced a progressive red shift in the center of mass of the intrinsic fluorescence emission spectrum, with a maximum displacement of 650 cm )1 . HHP (270 MPa) shifted the centre ofmass by only 370 cm )1 .HHP combined with a subdenaturing urea concentration (1.5M) displaced the centre of mass by
750 cm )1 ....

8p dell39 03-04-2013 31 3   Download

AChRe )⁄ ) mice lacke-subunits of the acetylcholine receptor and thus fail to express adult-type receptors. The expression of fetal-type receptors throughout postnatal life alters postsynaptic signal transduction and causes a fast-to-slow fiber type transition, both in slow-twitch soleus muscle and in fast-twitch extensor digitorum longus muscle.

13p media19 06-03-2013 31 3   Download

l-Rhamnose isomerase (l-RhI) catalyzes the reversible isomerization of l-rhamnose to l-rhamnulose. Pseudomonas stutzeril-RhI, with a broad substrate specificity, can catalyze not only the isomerization ofl-rhamnose, but also that betweend-allose andd-psicose. For the aldose–ketose isomer-ization by l-RhI, a metal-mediated hydride-shift mechanism has been proposed, but the catalytic mechanism is still not entirely understood.

13p mobifone23 18-01-2013 58 4   Download

Do the Standards go far enough? Current position and focus of Standards versus what we now know regarding component matching. For some time now we have had ratified standards throughout the world relating to generic, or structured cabling. We have seen progress from the initial Category 3 cable and connectors, to Category 4 albeit very briefly, to Category 5 where we are today. We have also seen a shift of emphasis from component specifications to link and channel specifications which is somewhat more meaningful to the end user. In more recent times we have seen many drafts of Cat.

4p qd-tek 28-04-2010 98 8   Download