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Phöông Phaùp Ñieän Di<br /> <br /> (Electrophoresis)<br /> PGS.TS. Nguyeãn Ñöùc Tuaán<br /> <br /> Boä moân Phaân Tích – Kieåm Nghieäm<br /> Khoa Döôïc – Ñaïi hoïc Y Döôïc TPHCM<br /> <br /> Nguyeãn Ñöùc Tuaán<br /> <br /> Ñaïi hoïc Y Döôïc TPHCM<br /> <br /> Phöông Phaùp Ñieän Di<br /> Muïc tieâu<br /> <br /> - Hieåu ñöôïc nguyeân taéc hoaït ñoäng cuûa ñieän di mao quaûn<br /> Daøn baøi<br /> <br /> - Lòch söû<br /> - Ñònh nghóa<br /> - Phaân loaïi<br /> - Ñieän di mao quaûn<br /> <br /> Nguyeãn Ñöùc Tuaán<br /> <br /> Ñaïi hoïc Y Döôïc TPHCM<br /> <br /> Lòch söû<br /> 1791<br /> 1877<br /> 1897<br /> 1897<br /> 1923<br /> 1930<br /> 1939<br /> 1950<br /> 1955<br /> 1958<br /> 1967<br /> 1967<br /> 1969<br /> 1969<br /> 1970<br /> 1970<br /> 1972<br /> 1979<br /> 1981<br /> <br /> Faraday<br /> Helmholtz<br /> Nernst<br /> Kohlrausch<br /> Kendall, Crittenden<br /> Tiselius<br /> Svenson<br /> Haglund, Tiselius<br /> Smithies<br /> Hjertén<br /> Martin, Everaerts<br /> Hjertén<br /> Giddings<br /> Virtanen<br /> Everaerts,<br /> Arlinger, Routs<br /> Verheggen<br /> Mikkers<br /> Jorgenson<br /> <br /> 1984 Terabe<br /> 1991 Jandik, Jones<br /> 1991 Knox<br /> <br /> Nguyeãn Ñöùc Tuaán<br /> <br /> Laws of Electrolysis<br /> Charged Solvent Layer Closed to Surface of a Wall<br /> Properties of Small Ions<br /> Kohlrausch Function describing the Order of Migration of Ions and their Concentration<br /> Rare Earth Metal Separation by "Ion Migraion Method"<br /> Thesis: Moving Boundary Method for Electrophoresis of Proteins (Nobel Price 1948)<br /> Development of Zone and Displacement Electrophoresis<br /> Electrophoresis Tube filled with Glass Beads and Glass Powder<br /> Gel Electrophoresis<br /> Electrophoresis in Free Solution<br /> Displacement Electrophoresis in Glass Tube with Hydroxyethylcellulose<br /> Elimination of Electroosmosis by Coating of Glass Tubes<br /> Non-Diffusional Model of Concentration Distribution in Free Zone Electrophoresis<br /> Glass Capillaries 0.2 - 0.5 mm I.D.<br /> Capillary Isotachophoresis<br /> UV-Detection<br /> Conductivity Detection<br /> Use of High Voltage and TEFLON Capillaries<br /> Use of 75 µm I.D. Open Tubular Glass Capillaries:<br /> "High Performance Capillary Electrophoresis – HPCE"<br /> Combination of electrophoretic and chromatographic Separation:<br /> "Micellar Electrokinetic Capillary Chromatography – MECC"<br /> Use of Surface Active Electrolyte Additives for Reversal of Electroosmotic Flow<br /> "Capillary Electrochromatography – CEC"<br /> <br /> Ñaïi hoïc Y Döôïc TPHCM<br /> <br /> Anion höõu cô vaø voâ cô<br /> 4<br /> <br /> Standard<br /> 1...thiosulfate<br /> <br /> 16..phosphite<br /> <br /> 2...bromide<br /> <br /> 17..chlorite<br /> <br /> 3...chloride<br /> <br /> 18..galacturate<br /> <br /> 4...sulfate<br /> <br /> 19..carnonate<br /> <br /> 5...nitrite<br /> 6...nitrate<br /> <br /> 20..acetate<br /> 21..ethanesulfonate<br /> <br /> 7...molybdate<br /> <br /> 22..propionate<br /> <br /> 8...azide<br /> 9...tungstate<br /> <br /> 23..propanesulfonate<br /> 24..butyrate<br /> <br /> 3<br /> 5<br /> <br /> 8<br /> 67<br /> 2<br /> 1<br /> <br /> 13<br /> 9<br /> <br /> 10<br /> 10<br /> <br /> 14<br /> <br /> 11<br /> <br /> 16<br /> 17<br /> 15<br /> <br /> 20<br /> <br /> 18<br /> <br /> 22<br /> 19<br /> <br /> 21<br /> <br /> 12<br /> <br /> 1.5min<br /> <br /> Nguyeãn Ñöùc Tuaán<br /> <br /> 23<br /> <br /> 24<br /> 25<br /> <br /> 26<br /> 28<br /> 27<br /> <br /> 89 seconds<br /> <br /> 30<br /> 29<br /> <br /> 3.1min<br /> <br /> 10.fluorophosphate 25..butyrsulfonate<br /> 11..chlorate<br /> 26..valerate<br /> 12..citrate<br /> 27..benzoate<br /> 13..fluoride<br /> 28..glutamate<br /> 14..formate<br /> 29..pentanesulfonate<br /> 15..phospahate<br /> 30..D-gluconate<br /> <br /> Ñaïi hoïc Y Döôïc TPHCM<br /> <br /> Ñònh nghóa<br />  Quaù trình taùch caùc tieåu phaân ñaõ ion hoùa vaø hoøa tan hay<br /> phaân taùn trong dung dòch ñieän giaûi döôùi taùc duïng cuûa ñieän<br /> tröôøng<br />  Ñoä dòch chuyeån ñieän di (linh ñoä ñieän di: electrophoretic<br /> mobility, EP) phuï thuoäc:<br />  Baûn chaát tieåu phaân: hình daïng, kích thöôùc, ñieän tích<br />  Dung dòch ñieän giaûi: baûn chaát, noàng ñoä, pH, ñoä nhôùt,….<br /> <br /> Nguyeãn Ñöùc Tuaán<br /> <br /> Ñaïi hoïc Y Döôïc TPHCM<br /> <br />