6 N G NGHE - MOI TRl/dNG<br />
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LIEU LlftfXG ENZYME ROHAPECT®<br />
im. THICH HttU fflE TACH LCfP I\HO?T<br />
C^UJ Ai HAT CA PHE TRONG CHE BIEN<br />
EO PHlftfNG P H A P iTeJT<br />
TS Nguy in Vdn Thudng<br />
Vien KHKT Ndng ldm nghiep Tdy Nguyen<br />
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l.GIOn THIEU tach nhdt pho bien hien nay la len men tu nhien va<br />
Trong che bien ca phe bang phuong phap udt, danh nhdt eo hgc. Ca hai phuong phap nay d8u co<br />
giai doan tach Idp nhdt nam tren vo thoc sau giai nhiing bat lgi nhat dinh, va de tranh dugc nhiing han<br />
doan xat bo vo qua va trudc giai doan phoi say dong che ciia hai phuong phap, cae nha khoa hgc da de<br />
vai tro rat quan trgng va c6 anh hudng nbi6u din xuat nghien ciiu su dung enzyme de phan buy Idp<br />
chat lugng san pham sau cimg. Hai phuong phap nhdt ciia ca phe.<br />
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Galacturonic Acid<br />
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(M) Methanol<br />
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Hinh 1. Cdc enzyme phdn hiiy tgi khu vuc cdu true sudn thdng eua phdn tic pectin<br />
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Nghien eiiu ban dau cua chiing toi cho thay Phan Lan. Nhirng enzyme chinh trong che pham<br />
che pham Rohapect® 1OL c6 tac dung tot trong viec gom PG, PE va PL. Che pham dang long, mau nau,<br />
phan buy dich triet Idp nhdt cua ca phe voi. ty trgng 1,18 g/ml va boat lire t6i thieu 260 PA. PA la<br />
Rohapect® lOL duge san xuat bang giong nam gia tri nghich dao ciia khoi lugng enzyme tinh bing<br />
Aspegillus niger va la san pham cua Cong ty AB kg de pha buy hoan toan pectin trong 100 lit nudc ep<br />
Enzyme, trirc thuoc tap doan ABF Ingredients, trai tao trong dieu kien 50°C, pH 3,2, trong 1 gid.<br />
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BAN TIN THONG TIN KH&CN SO 04/2010<br />
KHOA HOC - CONG NGHE - MOI TRl/dNG<br />
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San pham dap iing yeu ciu cua Hgi d6ng Chuyen 2. PHU^ONG PHAP NGHIEN ClTU<br />
gia Hon hgp ciia FAO&WHO va Uy ban Codex v6 - Qua chin ca phe voi thu hoach thang 12 nam<br />
hoa chat thirc pham (FCC). 2009 tai Buon Ma Thugt dugc tach vo bang may xat<br />
De CO dii CO sd khuyen cao cho cac nha che trdng thong thudng de lay ra 90 kg ca phe thoc. Chia<br />
bien ca phe irng dung che phim Rohapect® 1 OL xu dSu ca phe thoc vao 30 chau (moi chau 3 kg) va<br />
ly Idp nhdt trong che bien udt, trudc bet can phai<br />
phan lam 6 nhom de xu ly enzyme Rohapect® lOL<br />
xac dinh dieu kien thich hgp nhu lieu lugng che<br />
tuong ling vdi 6 lieu lugng (tinh bang phan trieu<br />
pham, do pH va nhiet do moi trudng de che pham<br />
phan buy Idp nhdt dat ket qua tot nhat. Bai viet gidi ppm, w/w) (bang 1). Nam chau d moi nhom dugc<br />
thieu ket qua nghien ciiu xac dinh lieu lugng che bo sung nudc theo cac ti le ca phe/nudc (w/w) lan<br />
pham Rohapect® 1 OL thich hgp cho xir ly Idp nhdt lugt la 10/5, 10/4, 10/3, 10/2 va 10/1. Dao trgn ca<br />
ca phe voi (Robusta) trong thdi gian 2 gid. phe vdi enzyme ky ludng va u trong 2 gid.<br />
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Bang 1. Cac cong thuc xu ly enzyme Rohapect®10L<br />
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Cong thuc Lieu luong enzyme Ti le ca phe/nuffc<br />
I 100 ppm 10/5 10/4 10/3 10/2 10/1<br />
II 150 ppm 10/5 10/4 10/3 10/2 10/1<br />
II 200 ppm 10/5 10/4 10/3 10/2 10/1<br />
IV 250 ppm 10/5 10/4 10/3 10/2 10/1<br />
V 300 ppm 10/5 10/4 10/3 10/2 10/1<br />
VI 350 ppm 10/5 10/4 10/3 10/2 10/1<br />
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- Sau khi li 2 gid, kiem tra va danh gia miic do 3. KET QUA VA THAO LUAN<br />
sach nhdt. Miic do sach nhdt dugc danh gia nhu 3.1. Hieu qua tach lop nhot ca phe ciia<br />
sau: hat chua sach nhdt neu lugng nhdt con khoang Rohapect® lOLffcac lieu luong khac nhau<br />
20%; hat sach chap nhan dugc neu lugng nhdt eon Ket qua bang 2 eho thay d eac ti le ca<br />
khoang 15%; va hat sach nhdt neu lugng nhdt con phe/nudc khac nhau thi miic do sach nhdt cua ca<br />
dudi 10%. phe thoc deu tang dan theo lieu lugng enzyme. Ti le<br />
- Rira ca phe 3 lan bang 15 lit nudc sach/chau ca phe/nudc cang cao thi miic do sach nhdt cua ca<br />
va dem phoi kho. Xat bo vo thoc, can khoi lugng ca phe thoc cang cao. Ti le ca phe/nudc 10/1 co miic do<br />
phe nhan va tinh ti le % khoi lugng ca phe nhan kho sach nhdt kha cao va dang chii y la chi vdi lieu<br />
(am do 12,5%) so vdi khoi lugng ca phe thoc udt lugng enzyme 150 ppm thi ti le sach nhdt da dat tdi<br />
trudc khi xir ly enzyme. 95%. Ly giai ket qua nay nhu sau: vdi ti le ea<br />
- Lap lai thi nghiem 3 lan va lay ket qua trung phe/nudc cang cao, tiic lugng nudc dimg cang it, thi<br />
binh. So lieu dugc xu ly bang phan mem thong ke dung dich enzyme cang dam dac, do vay boat luc<br />
Minitab 12.21 for Windows. enzyme se cao hon va tac dung phan buy Idp nhdt se<br />
- Danh gia chat lugng tach theo phuong phap nhanh hon so vdi ti le ca phe/nudc thip (tiic dung<br />
cua Hiep hgi Ca phe Dac san Hoa ky. dich enzyme loang hon).<br />
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BAN TIN THONG TIN KH&CN SO 04/2010<br />
KHOA HOC - CONG NGHE - MOI TRl/dNG<br />
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Bang 2. Muc do sach nhot cua ca pheffcac lilu luong enzyme va ti le ca phe/nuffc<br />
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Muc do sach nhot cua ca phe thoc (%) theo ti le ca phe/nuffc<br />
Lieu luffng enzyme<br />
10/5 10/4 10/3 10/2 10/1 Trung binh<br />
100 ppm 65 65 65 75 85 71<br />
150 ppm 70 70 70 80 95 77<br />
200 ppm 75 80 80 85 95 83<br />
250 ppm 80 80 80 90 95 85<br />
300 ppm 85 85 85 95 100 90<br />
350 ppm 90 90 90 95 100 93<br />
Trung binh 77 77 78 87 95 -<br />
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3.2. Anh huong ciia lieu luong Rohapect® lOL mong mot phan vo thoc cang ldn, giup mot so hgp<br />
toi khoi luong san pham chat boa tan tir trong hat thoat ra khoi vo th6c de<br />
Ti le ca phe nhan thu dugc giam dan theo su hon, vi vay khoi lugng nhan giam cang nhieu. Tuy<br />
tang eua lieu lugng enzyme Rohapect® 1 OL tir 150- vay su chenh lech ve ti le ca phe nhan giiia cac cong<br />
350 ppm (bang 3). Dieu nay dugc giai thich la khi thuc xir ly lieu lugng enzyme khac nhau khong co y<br />
xir ly enzyme vdi lieu lugng cang cao, Idp nhdt nghia thong ke (p>0.05).<br />
cang bi phan buy nhanh va kha nang enzyme lam<br />
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Bang 3. Ti le ca phe nhan khoffcac lieu luong enzyme va ti le ca phe/nuffc<br />
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Lieu lirffng % (w/w) ca phe nhan thu hoi theo ti le ca phe/nudc<br />
enzyme 10/5 10/4 10/3 10/2 10/1 Trung binh<br />
100 ppm 33,5 ±0,2 33,7 ±0,2 33,8 ±0,1 34,9 ± 0,2 36,3 ± 0,2 34,4<br />
150 ppm 33,4 ±0,8 33,4 ± 0,2 32,8 ± 0,2 34,5 ± 0,2 36,0 ±0,2 34,0<br />
200 ppm 33,1 ±0,9 33,1 ±0,3 33,0 ±0,3 34,3 ±0,1 i 35,7 ±0,2 i 33,8<br />
\ 35,6 ±0,11<br />
33,1 ±0,2 1 33,4<br />
250 ppm i 32,8 ±0,9 32,7 ±0,4 32,6 ±0,3<br />
300 ppm i 32,6 ±0,1 32,5 ±3,0 32,2 ± 0,5 32,8 ±0,1 1 35,3 ±0,0 33,1<br />
350 ppm 31,2 ±0,7 31,6 ±0,8 31,0 ±0,5 32,6 ±0,2 ! 35,0 ±0,2 32,3<br />
32,6 1<br />
1 33,7 I 35,6 [<br />
Trung binh | 32,8 32,8<br />
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KSt qua ciing cho thiy khdi lugng ca phe Kk qua xii ly thong ke cho thiy khong co sir<br />
nhan thu dugc tang din theo su tang cua ti le ca Urong tac giira hai yeu to lieu lugng enzyme va ti le<br />
phe/nudc va su khac biet vg ti le ca phe nhan thu caphe/nudctditilecaphenhanthudugc(p>0.05).<br />
dugc CO y nghia thong ke (p ly 2 h<br />
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Rohapect<br />
150 ppT* ^<br />
Rohapect<br />
300 p p m<br />
X i i ' ly 2 h<br />
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Rohapect<br />
100 ppm<br />
Xu" ly 2h<br />
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Rohapect^<br />
250 p p m<br />
XiV ly 21 0 6 i oh U'ng<br />
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'- 2. }.4du sdc cd phi thoc kho a cdc eong tlnic lieu luong Rohapect khac nhau (Anh: VT)<br />
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BAN TIN THONG TIN KH&CN SO 04/2010<br />