Enzymatic

Peroxisomes are pivotal metabolic organelles that exist in almost all eukaryote cells. A reduction in numbers and enzymatic activities of peroxisomes was found in colon adenocarcinomas. However, the role of peroxisomes or the peroxisome pathway in colorectal cancer (CRC) is not defined.

17p vielonmusk 21-01-2022 11 0   Download

the results have shown that among twelve isolated bacterial strains, three strains were screened based on their enzymatic potential, and were later identified by 16S rRNA gene sequencing, i.e. showing that the strains belong to the genus Pseudomonas aeruginosa, and Bacillus wiedmannii with a similarity percentage of 99.33% and 100%, respectively, with their corresponding type of strains.

7p mudbound 10-12-2021 8 1   Download

In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NH4 )2 HPO4 as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45°C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h.

14p thiencuuchu 27-11-2021 18 2   Download

Nicotinamide N-methyltransferase (NNMT), a key cytoplasmic protein in the human body, is accountable to catalyze the nicotinamide (NCA) N1 -methylation through S-adenosyl-L-methionine (SAM) as a methyl donor, which has been linked to many diseases. Although extensive studies have concerned about the biological aspect, the detailed mechanism study of the enzyme function, especially in the part of protein dynamics is lacking.

9p thiencuuchu 27-11-2021 14 1   Download

The metabolic activities of the casein peptides were verified using purified enzymatic inhibition studies. The regulatory enzyme inhibition led us to conclude a positive correlation with the role of casein peptides in modulating the diabetic and hypertensive metabolic pathways in the biological system. Purified enzymes were used for the investigation to clearly observe the direct impact on the diabetes and hypertension related bioprocesses.

10p nguaconbaynhay7 15-08-2020 14 1   Download

The results of microbiological and toxicological tests have proved the safety of the bone-muscular cod waste (BMW). Waste (humerus with meat cuts) from cod cutting on fillets contains 18,95 % of a full-grade animal protein and insignificant amount of fat (0.15 %).

14p lucastanguyen 01-06-2020 16 2   Download

A pot culture experiment was conducted to study the effect of humic acid on soil microbial population and enzymatic activity in groundnut in an Alfisol at Main Agricultural Research Station, Dharwad.

5p gaocaolon5 14-06-2020 5 1   Download

Nghiên cứu nhằm xác định các điều kiện thủy phân sò lông thích hợp bằng các enzyme trên. Kết quả đã chỉ ra rằng điều kiện thích hợp cho Protamex ở giai đoạn đầu của quá trình thủy phân là tỷ lệ Protamex/nguyên liệu 0,3%, nhiệt độ thủy phân 500 độ C và thời gian thủy phân 4 giờ và điều kiện thủy phân thích hợp cho Flavourzyme như sau: Tỷ lệ Flavourzyme/ nguyên liệu 0,2%, nhiệt độ thủy phân 500 độ C và thời gian thủy phân 3 giờ.

7p vidanh27 08-12-2018 62 2   Download

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25–40 ◦C), enzyme concentration (0.1–3.0 g l−1), lactose concentration (0.0278–0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis–Menten kinetic model with inhibition by the product (galactose), which reveals that the substrate (lactose) and the product (galactose) present similar affinity for the active site of the enzyme.

10p abk1996 04-09-2018 31 0   Download

The purpose of this work was to study protein and lipid recovery from yellowfin tuna head by enzymatic hydrolysis as well as to analyse the amino acid compositions in tuna head protein hydrolysate and the fatty acid compositions in tuna head oil.

9p nganga_08 12-10-2015 79 3   Download

Susceptibility of annealed starches to hydrolysisbya-amylase and glucoamylase has many contents: preparation of annealed starch, enzymatic hydrolysis of starch granules, apparent amylose content, starch morphology, thermal properties, x-ray diffraction, enzymatic hydrolysis of starch granules,...

11p cscpubka 05-05-2014 71 2   Download

With the aim to distinguish between local and global conformational changes induced by trifluoroethanol in RNase A, spectroscopic and activity measurements in combination with proteolysis by unspecific proteases have been exploited for probing structural transitions of RNase A as a function of trifluoroethanol concentration. At 30% (v/v) trifluoroethanol (pH 8.0; 25 °C), circular dichroism and fluorescence spectroscopy indicate a cooperative collapse of the tertiary structure of RNase A coinciding with the loss of its enzymatic activity....

7p system191 01-06-2013 44 2   Download

This review describes studies of particular enzymatically catalyzed reactions to investigate the possibility that catalysis is mediated by protein dynamics. That is, evolution has crafted the protein backbone of the enzyme to direct vibrations in such a fashion to speed reaction. The review presents the theoretical approach we have used to investigate this problem, but it is designed for the nonspecialist.

10p system191 01-06-2013 33 3   Download

An exo-1,3-b-glucanase has been isolated from cultural filtrate of Trichoderma viride AZ36. The N-terminal sequence of the purified enzyme (m ¼ 61 ^ 1 kDa) showed no significant homology to other known glucanases. The 1,3-b-glucanase displayed high activity against laminarins, curdlan, and 1,3-b-oligoglucosides, but acted slowly on 1,3-1,4-b-oligoglucosides. No significant activity was detected against high molecular mass 1,3-1,4-bglucans. The enzyme carried out hydrolysis with inversion of the anomeric configuration.

9p system191 01-06-2013 42 5   Download

The calcineurin (CaN) a and b catalytic subunit isoforms are coexpressed within almost all cell types. The enzymatic properties of CaN heterodimers comprised of the regulatory B subunit (CnB) with either the a or b catalytic subunit were compared using in vitro phosphatase assays. CaN containing the a isoform (CnAa) has lower Km and higher Vmax values than CaN containing the b isoform (CnAb) toward the PO4RII, PO4-DARPP-32(20–38) peptides, and p-nitrophenylphosphate (pNPP).

9p research12 01-06-2013 23 2   Download

This study investigated the enzymatic function of two putative plant GPXs, GPXle1 from Lycopersicon esculentum and GPXha2 from Helianthus annuus, which show sequence identities with the mammalian phospholipid hydroperoxide glutathione peroxidase (PHGPX). Both purified recombinant proteins expressed in Escherichia coli show PHGPX activity by reducing alkyl, fatty acid and phospholipid hydroperoxides but not hydrogen peroxide in the presence of glutathione.

7p research12 01-06-2013 35 3   Download

We previously demonstrated that the major ecto-nucleoside triphosphate phosphohydrolase in the chicken liver membranes is an ecto-ATP-diphosphohydrolase (ectoATPDase) [Caldwell, C., Davis, M.D. & Knowles, A.F. (1999) Arch. Biochem. Biophys. 362, 46–58]. Enzymatic properties of the liver membrane ecto-ATPDase are similar to those of the chicken oviduct ecto-ATPDase that we have previously purified and cloned. Using antibody developed against the latter, we have purified the chicken liver ecto-ATPDase to homogeneity. ...

10p research12 01-06-2013 37 4   Download

In order to study some of its enzymatic properties, phosphatidylinositol synthase 1 (AtPIS1) from the plant Arabidopsis thaliana was expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data clearly show that the CDP-diacylglycerol-independent exchange reaction can occur using endogenous PtdIns molecular species or PtdIns molecular species from soybean added exogenously....

6p research12 01-06-2013 29 4   Download

Nuclear receptors (NRs) are ligand-regulated, DNA-binding transcription factors that function in the chromatin environment of the nucleus to alter the expression of subsets of hormone-responsive genes. It is clear that chromatin, rather than being a passive player, has a profound effect on both transcriptional repression and activation mediated by NRs.

9p research12 01-06-2013 32 2   Download

By replacing specific amino acids at positions 112, 147 and 152 of the human aldosterone synthase (CYP11B2) with the corresponding residues from human, mouse or rat 11b-hydroxylase (CYP11B1), we have been able to investigate whether these residues belong to structural determinants of individual enzymatic activities. When incubated with 11-deoxycorticosterone (DOC), the 11b-hydroxylation activity of the mutants was most effectively increased by combining D147E and I112P (sixfold increase).

10p research12 01-06-2013 33 4   Download