Báo cáo khoa học: Aspartate transcarbamylase from the hyperthermophilic archaeonPyrococcus abyssi Insights into cooperative and allosteric mechanisms

Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi Insights into cooperative and allosteric mechanisms Sigrid Van Boxstael1, Dominique Maes2,3 and Raymond Cunin1 1 Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium 2 Ultrastructuur, Vrije Universiteit Brussel, Belgium 3 Vlaams Interuniversitair Instituut voor Biotechnologie, Belgium Keywords allostery; aspartate transcarbamylase; cooperativity; inhibition by analogues; Pyrococcus abyssi Correspondence R. Cunin, Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium Fax: 32 2 629 1473 Tel: 32 2 629 1341 E-mail: rcunin@vub.ac.be (Received 15 December 2004, revised 1 March 2005, accepted 22 March 2005) doi:10.1111/j.1742-4658.2005.04678.x Aspartate transcarbamylase (ATCase) (EC 2.1.3.2) from the hyperthermophilic archaeon Pyrococcus abyssi was purified...