Báo cáo khoa học: Deamidation of labile asparagine residues in the autoregulatory sequence of human phenylalanine hydroxylase

Two dimensional electrophoresis has revealed a micro-heterogeneity in the recombinant human phenylalanine hydroxylase (hPAH) protomer,that is the result of sponta-neous nonenzymatic deamidations of labile asparagine (Asn) residues [Solstad,T. and Flatmark,T. (2000)Eur. J. Biochem.267,6302–6310]. Using of a computer algorithm, the relative deamidation rates of all Asn residues in hPAH havebeenpredicted,andwehere verify thatAsn32,followed by a glycine residue,as well as Asn28 and Asn30 in a loop region of the N-terminal autoregulatory sequence (residues 19–33) of wt-hPAH,are among the susceptible residues....