Báo cáo khoa học: Expression, puriﬁcation and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:7

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Recently we have described a novel secreted protein (the WFIKKN protein) that consists of multiple types of prote-ase inhibitory modules, including two tandem Kunitz-type protease inhibitor-domains. On the basis of its homologies we have suggested that the WFIKKN protein is a multi-valent protease inhibitor that may control the action of different proteases. In the present work we have expressed the second Kunitz-type protease inhibitor domain of the human protein WFIKKN inEscherichia coli, purified it by affinity chromatography on trypsin-Sepharose and its structure was characterized by CD spectroscopy....

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