
Báo cáo khoa học: Kinetically controlled refolding of a heat-denatured hyperthermostable protein
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The thermal denaturation of endo-b-1,3-glucanase from the hyperthermo-philic microorganismPyrococcus furiosuswas studied by calorimetry. The calorimetric profile revealed two transitions at 109 and 144C, correspond-ing to protein denaturation and complete unfolding, respectively, as shown by circular dichroism and fluorescence spectroscopy data.
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