Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:8

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Duckd2-crystallin is a soluble tetrameric lens protein. In the presence of guanidinium hydrochloride (GdnHCl),it undergoes stepwise dissociation and unfolding. Gel-filtra-tion chromatography and sedimentation velocity analysis has demonstrated the dissociation of the tetramer protein to a monomeric intermediate with a dissociation constant of 0.34lM 3 . Dimers were also detected during the dissociation and refoldingprocesses.The sharpenhancement of 1-anilino naphthalene-8-sulfonic acid (ANS) fluorescence at 1M GdnHCl strongly suggested that the dissociated monomers were in a molten globule state under these conditions....

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