Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products

Pronase (type XXV serine protease fromStreptomyces griseus) efficiently depolymerizes chitosan, a linearbfi1,4-linked polysaccharide of 2-amino-deoxyglucose and 2-amino-2-N-acetylamino-D-glucose, to low-molecular weight chitosans (LMWC), chito-oligomers (degree of poly-merization, 2–6) andmonomer. The maximumdepolymeri-zationoccurredatpH 3.5and37C,andthereactionobeyed Michaelis–Menten kinetics with aKmof 5.21 mgÆmL )1 and Vmaxof 138.55 nmolesÆmin )1 Æmg )1 .