
Báo cáo khoa học: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 fibroblasts
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The cellular level of the organic osmolyte taurine is abalance betweenactiveuptakeandpassive leakviaavolume sensitive pathway. Here, we demonstrate that NIH3T3 mouse fibro-blasts express a saturable, high affinity taurine transporter (TauT,Km¼18lM), and that taurine uptake via TauT is aNa+ -and Cl– -dependent process with an apparent 2.5 : 1 : 1 Na + /Cl – /taurine stoichiometry. Transport activ-ity is reduced following acute administration of H2O2 or activatorsof proteinkinasesAorC.
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