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Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae Identification of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:15

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Structural elucidation of the lipopolysaccharide (LPS) from three nontypeable Haemophilus influenzaeclinical isolates, 1209, 1207 and 1233 was achieved using NMR spectro-scopy and ESI-MS on O-deacylated LPS and core oligo-saccharide (OS) material as well as ESI-MS n on permethylated dephosphorylated OS. It was found that the organisms expressed a tremendous heterogeneous glyco-form mixture resulting from the variable length of the OS chains attached to the common structural element of H. influenzae, L-a-D–Hepp-(1fi2)-[PEtnfi6]-L-a-D–Hepp-(1fi3)-[b-D-Glcp-(1fi4)]-L-a-D–Hepp-(1fi5)-[PPEtnfi4]-a-Kdop-(2fi6)-Lipid A. ...

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Nội dung Text: Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae Identification of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates

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