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Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:7

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Important hydrogenbonding interactions between substrate OH-groups inyeasta-glucosidases andoligo-1,6-glucosidase from glycoside hydrolase family 13 have been identi®ed by measuring the rates of hydrolysis of methyla-isomaltoside and its seven monodeoxygenated analogs. The transition-state stabilization energy, DDGà, contributed by the indi-vidual OH-groups was calculated from the activities for the parent and the deoxy analogs, respectively, according toDDGà ˆ±RTln[(Vmax/Km)analog /(Vmax/Km) parent]....

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Nội dung Text: Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides

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