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Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:12

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Self-regulation of the 2-oxo acid dehydrogenase complexes during catalysiswas studied.Radical species as sideproducts of catalysis were detected by spin trapping, lucigenin fluor-escence and ferricytochromec reduction. Studies of the complexes after converting the bound lipoate or FAD cofactors tononfunctional derivatives indicated that radicals are generated viaFAD. In the presence of oxygen, the 2-oxo acid, CoA-dependent production of the superoxide anion radical was detected.

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Nội dung Text: Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species

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